Sample Requirement:

  • Gel band of SDS-PAGE
    • Gel size ≤ 3 x 8 x 1.5 mm
  • Protein extracts
    • Total amount ≥ 1 µg (Basic analysis), 20 µg (Standard analysis)
    • Protein concentration ≥ 0.1 mg/mL (Basic analysis), 0.5 mg/mL (Standard analysis)

Recommended Analysis:

  • Basic: Gel band, Purified proteins, Fractionation.
  • Standard: Cell lysate, Tissue extracts, or Body fluid with abundant proteins depletion

Turnaround time: 10 days (Basic), 15 days (Standard)

脫色- 將蛋白質呈色染劑去除
水解- 雙硫鍵還原及蛋白酶水解
比對- 依物種進行資料庫比對
萃取- 萃取胜肽並進行質譜分析